Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Application of CRISPR/Cas9-Based Reverse Genetics in Leishmania braziliensis: Conserved Roles for HSP100 and HSP23.

Genes - 30 Sept 2020

Adaui Vanessa, Kröber-Boncardo Constanze, Brinker Christine, Zirpel Henner, Sellau Julie, Arévalo Jorge, Dujardin Jean-Claude, Clos Joachim

Abstract excerpt

The protozoan parasite Leishmania (Viannia) braziliensis (L. braziliensis) is the main cause of human tegumentary leishmaniasis in the New World, a disease affecting the skin and/or mucosal tissues. Despite its importance, the study of the unique biology of L. braziliensis through reverse genetics analyses has so far lagged behind in comparison with Old World Leishmania spp. In this study, we successfully applied...

Topics

Join the communities discussing this publication.

Heat-Shock Proteins
Endopeptidase Clp
Gene Editing
Gene Targeting
Genes, Protozoan

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Leishmania major

Reverse Genetics

Thermotolerance

Source

Open in PubMed Open full text

Identifiers

PMID: 33007987
DOI: 10.3390/genes11101159
PMCID: PMC7601497

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Application of CRISPR/Cas9-Based Reverse Genetics in Leishmania braziliensis: Conserved Roles for HSP100 and HSP23.PMID 33007987Read ↗

Application of CRISPR/Cas9-Mediated Genome Editing in Leishmania.Semantic proximity Read ↗

Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair MechanismsSemantic proximity Read ↗

First efficient CRISPR-Cas9-mediated genome editing in L eishmania parasitesSemantic proximity Read ↗

CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1Semantic proximity Read ↗

Recent advances in Leishmania reverse genetics: Manipulating a manipulative parasiteSemantic proximity Read ↗

A Second Generation Leishmanization Vaccine with a Markerless Attenuated <i>Leishmania major</i> Strain using CRISPR gene editingSemantic proximity Read ↗

A novel <i>Leishmania infantum</i> reference strain for gene editing and the study of visceral leishmaniasisSemantic proximity Read ↗

CRISPR-Cas9-Mediated Genome Editing in Leishmania donovaniSemantic proximity Read ↗

Novel CRISPR-based detection of Leishmania speciesSemantic proximity Read ↗

Genetically Validated Drug Targets in Leishmania: Current Knowledge and Future ProspectsSemantic proximity Read ↗

A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editingSemantic proximity Read ↗

Generation and Characterization of a Dual-Reporter Transgenic Leishmania braziliensis Line Expressing eGFP and LuciferaseSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.