Article
Development of amber suppressor tRNAs appropriate for incorporation of nonnatural amino acids.
Nucleic acids symposium series (2004) - 1 Jan 2006
Taira Hikaru, Matsushita Yohsuke, Kojima Kenji, Hohsaka Takahiro
Abstract excerpt
An amber suppression method has been used for incorporation of nonnatural amino acids into proteins. However, the incorporation efficiency of nonnatural amino acids through an amber codon has been low, which restricts the application of the proteins containing nonnatural amino acids. In this study, we screened a wide variety of amber suppressor tRNAs to discover tRNAs capable to incorporate nonnatural amino acids...
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