Article
Incorporation of non-canonical amino acids into proteins in yeast.
Fungal genetics and biology : FG & B - 1 Apr 2016
Wiltschi Birgit
Abstract excerpt
Non-canonical amino acids add extraordinary chemistries to proteins when they gain access to translation. In yeast, they can be incorporated into proteins by replacing a canonical amino acid or in a site-specific manner in response to an amber stop codon. The first approach simply exploits the natural substrate tolerance of the aminoacyl-tRNA synthetases in an auxotrophic host. The latter requires the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
