Engineering of tRNAPro1E2 anticodon stem enhances multiple/consecutive ribosomal incorporation of N-methyl-l-α-amino acids and d-α-amino acids.

Abstract excerpt

Transfer RNA (tRNA) structures influence the incorporation efficiency of amino acids, particularly when nonproteinogenic amino acids (npAAs), such as N-methyl-l-α-amino acids (MeAAs) and d-α-amino acids (d-AAs), are charged. Such npAAs are generally far poorer substrates than the 20 canonical α-amino acids for translation. However, we have shown that their incorporation efficiencies could be improved by using a...

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