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Article

Amber codon-mediated expanded saturation mutagenesis of proteins using a cell-free translation system.

Journal of bioscience and bioengineering - 1 Jun 2012

Shozen Naoki, Watanabe Takayoshi, Hohsaka Takahiro

Abstract excerpt

Saturation mutagenesis of proteins, in which an amino acid at a specific site is substituted with each of the other 19 amino acids, is a powerful method for protein analysis and engineering. However, 19 mutated genes have to be prepared to express all possible amino acid-substituted proteins at one site. We previously reported a four-base codon-mediated saturation mutagenesis method for the expression of all 20...

Topics

Amino Acid Substitution
Amino Acids
Cell-Free System
Codon
Codon, Terminator
Escherichia coli
Ligands
Mutagenesis
Mutation
Protein Biosynthesis
Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Transfer

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Identifiers

PMID: 22365415
DOI: 10.1016/j.jbiosc.2012.01.018

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