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Article

Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells.

Nucleic acids research - 21 Jun 2021

Bartoschek Michael D, Ugur Enes, Nguyen Tuan-Anh, Rodschinka Geraldine, Wierer Michael, Lang Kathrin, Bultmann Sebastian

Abstract excerpt

The genetic code of mammalian cells can be expanded to allow the incorporation of non-canonical amino acids (ncAAs) by suppressing in-frame amber stop codons (UAG) with an orthogonal pyrrolysyl-tRNA synthetase (PylRS)/tRNAPylCUA (PylT) pair. However, the feasibility of this approach is substantially hampered by unpredictable variations in incorporation efficiencies at different stop codon positions within target...

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PMID: 33684219
DOI: 10.1093/nar/gkab132
PMCID: PMC8216290

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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