Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code.

doi:10.1371/journal.pone.0031992

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Article

Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code.

PloS one - 1 Jan 2012

Nehring Sebastian, Budisa Nediljko, Wiltschi Birgit

Abstract excerpt

BACKGROUND: The suppression of amber stop codons with non-canonical amino acids (ncAAs) is used for the site-specific introduction of many unusual functions into proteins. Specific orthogonal aminoacyl-tRNA synthetase (o-aaRS)/amber suppressor tRNA(CUA) pairs (o-pairs) for the incorporation of ncAAs in S. cerevisiae were previously selected from an E. coli tyrosyl-tRNA synthetase/tRNA(CUA) mutant library....

Topics

Amino Acyl-tRNA Synthetases
Cloning, Molecular
Codon, Terminator
Escherichia coli
Eukaryota
Genetic Code
Genetic Engineering
Genetic Vectors
Genomic Library
Humans
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Biosynthesis

RNA, Transfer

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Superoxide Dismutase

Superoxide Dismutase-1

Tyrosine

Source

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Identifiers

PMID: 22493661
DOI: 10.1371/journal.pone.0031992
PMCID: PMC3320878

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