Article
Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion.
Nucleic acids research - 1 Jun 2010
Iraha Fumie, Oki Kenji, Kobayashi Takatsugu, Ohno Satoshi, Yokogawa Takashi, Nishikawa Kazuya, Yokoyama Shigeyuki, Sakamoto Kensaku
Abstract excerpt
Non-natural amino acids have been genetically encoded in living cells, using aminoacyl-tRNA synthetase-tRNA pairs orthogonal to the host translation system. In the present study, we engineered Escherichia coli cells with a translation system orthogonal to the E. coli tyrosyl-tRNA synthetase (TyrRS)-tRNA(Tyr) pair, to use E. coli TyrRS variants for non-natural amino acids in the cells without interfering with...
Topics
- Azides
- Escherichia coli
- Gene Deletion
- Genetic Code
- Genetic Complementation Test
- Methanococcales
- Monoiodotyrosine
- Mutation
- Protein Biosynthesis
- Protein Engineering
- RNA, Transfer, Tyr
- Saccharomyces cerevisiae
- Suppression, Genetic