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Article

Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion.

Nucleic acids research - 1 Jun 2010

Iraha Fumie, Oki Kenji, Kobayashi Takatsugu, Ohno Satoshi, Yokogawa Takashi, Nishikawa Kazuya, Yokoyama Shigeyuki, Sakamoto Kensaku

Abstract excerpt

Non-natural amino acids have been genetically encoded in living cells, using aminoacyl-tRNA synthetase-tRNA pairs orthogonal to the host translation system. In the present study, we engineered Escherichia coli cells with a translation system orthogonal to the E. coli tyrosyl-tRNA synthetase (TyrRS)-tRNA(Tyr) pair, to use E. coli TyrRS variants for non-natural amino acids in the cells without interfering with...

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Identifiers

PMID: 20159998
DOI: 10.1093/nar/gkq080
PMCID: PMC2887954

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