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Article

Inverting enantioselectivity of Burkholderia gladioli esterase EstB by directed and designed evolution.

Journal of biotechnology - 30 Mar 2007

Ivancic Mirela, Valinger Goran, Gruber Karl, Schwab Helmut

Abstract excerpt

Esterase EstB from Burkholderia gladioli, showing moderate S-enantioselectivity (E(S)=6.1) in the hydrolytic kinetic resolution of methyl-beta-hydroxyisobutyrate, was subjected to directed evolution in order to reverse its enantioselectivity. After one round of ep-PCR, saturation mutagenesis and high-throughput screening, it was found that different mutations at position 152 (in the vicinity of the active site)...

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Identifiers

PMID: 17147964
DOI: 10.1016/j.jbiotec.2006.10.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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