Article
Enhancing the efficiency of directed evolution in focused enzyme libraries by the adaptive substituent reordering algorithm.
Chemistry (Weinheim an der Bergstrasse, Germany) - 27 Apr 2012
Feng Xiaojiang, Sanchis Joaquin, Reetz Manfred T, Rabitz Herschel
Abstract excerpt
Directed evolution is a broadly successful strategy for protein engineering in the quest to enhance the stereoselectivity, activity, and thermostability of enzymes. To increase the efficiency of directed evolution based on iterative saturation mutagenesis, the adaptive substituent reordering algorithm (ASRA) is introduced here as an alternative to traditional quantitative structure-activity relationship (QSAR)...
Topics
- Algorithms
- Amino Acid Sequence
- Aspergillus niger
- Directed Molecular Evolution
- Epoxide Hydrolases
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Engineering