Article
Using site-saturation mutagenesis to explore mechanism and substrate specificity in thiamin diphosphate-dependent enzymes.
The FEBS journal - 1 Dec 2013
Andrews Forest H, McLeish Michael J
Abstract excerpt
For almost 20 years, site-saturation mutagenesis (SSM) has been used to evolve stereoselective enzymes as catalysts for synthetic organic chemistry. Much of this work has focused on enzymes such as lipases and esterases, although the range is rapidly expanding. By contrast, using SSM to study enzyme mechanisms is much less common. Instead, site-directed mutagenesis is more generally employed, with a particular...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.