Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations.

Abstract excerpt

Rational design of enzymes with improved properties, such as enantioselectivity, usually focuses mutations within the substrate binding site. On the other hand, directed evolution of enzymes usually targets the entire protein and discovers beneficial mutations far from the substrate binding site. In this paper, we propose an explanation for this discrepancy and show that a combined approach--random mutagenesis...

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