Selective enrichment for temperature-sensitive secretion mutants of mammalian cells using plant lectin, concanavalin A.

doi:10.1007/BF01232457

Back to search

Article

Selective enrichment for temperature-sensitive secretion mutants of mammalian cells using plant lectin, concanavalin A.

Somatic cell and molecular genetics - 1 Jul 1990

Henry K W, Brown J L, McCracken A A

Abstract excerpt

The use of conditional mutants as a genetic approach to study protein secretion in mammalian cells requires the isolation of a large number of mutants. Because a procedure for the direct selection of mutants with secretion defects is not available, their isolation depends upon the selective enric...

Topics

Animals
Concanavalin A
Genetic Techniques
Liver Neoplasms, Experimental
Mutagenesis
Phenotype
Proteins
Rats
Temperature
Tumor Cells, Cultured

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.