Article
Selective enrichment for temperature-sensitive secretion mutants of mammalian cells using plant lectin, concanavalin A.
Somatic cell and molecular genetics - 1 Jul 1990
Henry K W, Brown J L, McCracken A A
Abstract excerpt
The use of conditional mutants as a genetic approach to study protein secretion in mammalian cells requires the isolation of a large number of mutants. Because a procedure for the direct selection of mutants with secretion defects is not available, their isolation depends upon the selective enrichment of mutant phenotypes in a cell population. We have devised an enrichment strategy in which rat hepatoma cells...
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