Article
Genotype-specific signal generation based on digestion of 3-way DNA junctions: application to KRAS variation detection.
Clinical chemistry - 1 Oct 2006
Amicarelli Giulia, Adlerstein Daniel, Shehi Erlet, Wang Fengfei, Makrigiorgos G Mike
Abstract excerpt
BACKGROUND: Genotyping methods that reveal single-nucleotide differences are useful for a wide range of applications. We used digestion of 3-way DNA junctions in a novel technology, OneCutEventAmplificatioN (OCEAN) that allows sequence-specific signal generation and amplification. We combined OCEAN with peptide-nucleic-acid (PNA)-based variant enrichment to detect and simultaneously genotype v-Ki-ras2 Kirsten rat...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.