Suppression Probe Enrichment for Highly Sensitive and Multiplexed Detection of RAS Mutations in Colorectal Cancer.

Abstract excerpt

Although RAS mutations are concentrated in specific hotspots, their clinical detection remains reliant on next-generation sequencing (NGS), which is hindered by high costs, long turnaround times, and substantial sample requirements. Herein, we report suppression probe enrichment (SPE), a thermodynamically driven low-frequency-mutation enrichment integrated with multiplex TaqMan probes for ultrasensitive detection...

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