Chemically induced CD4 mutants of a human T cell line. Evidence for dissociation between binding of HIV I envelope and susceptibility to HIV I infection and syncytia formation.

Abstract excerpt

This study describes the derivation of a series of mutants from the human leukemic cell line CEM using the frame shift mutagen Ethyl-methanesulfonate followed by negative selection with multiple treatments of OKT4A + C, and sorting into CD4-, CD4-dull, and CD4-intermediate mutants. These mutants express reduced CD4 levels ranging from 0 to 60% of the parental line. The mutants were analyzed by staining with a...

Topics

• CD4 Antigens
• CD4-Positive T-Lymphocytes
• Cell Fusion
• Cell Line
• Genetic Vectors
• HIV Envelope Protein gp120
• HIV-1
• Humans
• In Vitro Techniques
• Mutation