Article
Protection of CCRF-CEM human lymphoid cells from antifolates by retroviral gene transfer of variants of murine dihydrofolate reductase.
Cancer gene therapy - 1 Jan 2000
Mareya S M, Sorrentino B P, Blakley R L
Abstract excerpt
Expression of certain variants of dihydrofolate reductase (DHFR) in mammalian cells protects them from methotrexate. Retroviral transfer of the gene for such a variant DHFR into hematopoietic cells might permit selection of modified cells in vivo by antifolate administration or alleviate antifola...
Topics
- Animals
- Cloning, Molecular
- Drug Resistance, Neoplasm
- Enzyme Stability
- Escherichia coli
- Folic Acid Antagonists
- Gene Dosage
- Gene Expression Regulation, Neoplastic
- Gene Transfer Techniques
- Humans
- Methotrexate
- Mice
- Mutation
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
- Recombinant Proteins
- Retroviridae
- Tetrahydrofolate Dehydrogenase
- Trimetrexate