Article
Reduced susceptibility to HIV-1 infection of ethyl-methanesulfonate-treated CEM subclones correlates with a blockade in their protein kinase C signaling pathway.
Journal of immunology (Baltimore, Md. : 1950) - 15 Jun 1992
Hillman K, Qian J, Siegel J N, Roderiquez G, Blackburn R, Manischewitz J, Norcross M, Golding H
Abstract excerpt
We have described the isolation of chemically induced CEM subclones that express CD4 receptors and bind soluble gp120, yet show a markedly reduced susceptibility to infection with HIV-1. Two subclones were found to have an abnormal response to the protein kinase C (PKC) activator PMA. PMA treatme...
Topics
- Base Sequence
- CD4-Positive T-Lymphocytes
- Cell Compartmentation
- Cells, Cultured
- Clone Cells
- Cytoplasm
- DNA-Binding Proteins
- Ethers, Cyclic
- Ethyl Methanesulfonate
- Gene Expression
- HIV Infections
- HIV Long Terminal Repeat
- Humans
- In Vitro Techniques