Construction and characterization of a radio-iodinatable mutant of recombinant human CD4.

doi:10.1016/s0022-1759(97)00195-6

Article

Construction and characterization of a radio-iodinatable mutant of recombinant human CD4.

Journal of immunological methods - 29 Dec 1997

Ferrer M, Godbout K L, Sullivan B J, Austen D A, Sanderson C T, Kelley K C, Osburne M S, Harrison S C, van Schravendijk M R

Abstract excerpt

Recombinant soluble human CD4 (rsCD4) has been used in iodinated form to study the interaction of CD4 with its ligands. However, the utility of [125I]-rsCD4 is limited because rsCD4 is inefficiently iodinated and the iodinated protein is poorly active. The iodination properties of rsCD4 most like...

Topics

Animals
CD4 Antigens
HIV Envelope Protein gp120
Humans
Iodine Radioisotopes
Isotope Labeling
Mice
Mutation
Recombinant Proteins

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