Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction and characterization of a radio-iodinatable mutant of recombinant human CD4.

Journal of immunological methods - 29 Dec 1997

Ferrer M, Godbout K L, Sullivan B J, Austen D A, Sanderson C T, Kelley K C, Osburne M S, Harrison S C, van Schravendijk M R

Abstract excerpt

Recombinant soluble human CD4 (rsCD4) has been used in iodinated form to study the interaction of CD4 with its ligands. However, the utility of [125I]-rsCD4 is limited because rsCD4 is inefficiently iodinated and the iodinated protein is poorly active. The iodination properties of rsCD4 most likely reflect the poor accessibility of the tyrosine residues, apparent from the available X-ray structures. We have...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CD4 Antigens
Share to HIV Envelope Protein gp120

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Iodine Radioisotopes

Share to Mutation

Share to Recombinant Proteins

Source

Identifiers

PMID: 9520304
DOI: 10.1016/s0022-1759(97)00195-6

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 9 closest

Construction and characterization of a radio-iodinatable mutant of recombinant human CD4.PMID 9520304Read ↗

A rat CD4 mutant containing the gp120-binding site mediates human immunodeficiency virus type 1 infection.Semantic proximity Read ↗

A human mutant CD4 molecule resistant to HIV-1 binding restores helper T-lymphocyte functions in murine CD4-deficient mice.Semantic proximity Read ↗

High level expression in Chinese hamster ovary cells of soluble forms of CD4 T lymphocyte glycoprotein including glycosylation variants.Semantic proximity Read ↗

Chemically induced CD4 mutants of a human T cell line. Evidence for dissociation between binding of HIV I envelope and susceptibility to HIV I infection and syncytia formation.Semantic proximity Read ↗

Identification of critical amino acids for 3,5,3'-triiodothyronine deiodination by human type 1 deiodinase based on comparative functional-structural analyses of the human, dog, and rat enzymes.Semantic proximity Read ↗

1.7 A structure of the stabilized REIv mutant T39K. Application of local NCS restraints.Semantic proximity Read ↗

Development of recombinant human prolactin receptor antagonists by molecular mimicry of the phosphorylated hormone.Semantic proximity Read ↗

A monoclonal antibody with thyrotropin (TSH) receptor inverse agonist and TSH antagonist activities binds to the receptor hinge region as well as to the leucine-rich domain.Semantic proximity Read ↗

The role of the active site cysteine in catalysis by type 1 iodothyronine deiodinase.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.