Article
Multiple K-ras codon 12 mutations in cholangiocarcinomas demonstrated with a sensitive polymerase chain reaction technique.
Cancer research - 1 Jul 1991
Levi S, Urbano-Ispizua A, Gill R, Thomas D M, Gilbertson J, Foster C, Marshall C J
Abstract excerpt
By using a modified polymerase chain reaction strategy, we have devised an approach to detect a K-ras oncogene mutated at codon 12 in the presence of 1000 normal alleles. This is a considerable improvement in sensitivity on previous assays. Application of this assay to 15 cholangiocarcinomas show...
Topics
- Adenoma, Bile Duct
- Adenomatous Polyposis Coli
- Base Sequence
- Codon
- Genes, ras
- Humans
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Proto-Oncogene Proteins p21(ras)
- Tumor Cells, Cultured