Article
Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b.
Virology - 1 Aug 2006
Murakami Kyoko, Ishii Koji, Ishihara Yousuke, Yoshizaki Sayaka, Tanaka Keiko, Gotoh Yasufumi, Aizaki Hideki, Kohara Michinori, Yoshioka Hiroshi, Mori Yuichi, Manabe Noboru, Shoji Ikuo, Sata Tetsutaro, Bartenschlager Ralf, Matsuura Yoshiharu, Miyamura Tatsuo, Suzuki Tetsuro
Abstract excerpt
We show that a dicistronic hepatitis C virus (HCV) genome of genotype 1b supports the production and secretion of infectious HCV particles in two independent three-dimensional (3D) culture systems, the radial-flow bioreactor and the thermoreversible gelation polymer (TGP), but not in monolayer cultures. Immunoreactive enveloped particles, which are 50-60 nm in diameter and are surrounded by membrane-like...
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