Article
Mutations in the aphA-2 gene of transposon Tn5 mapping within the regions highly conserved in aminoglycoside-phosphotransferases strongly reduce aminoglycoside resistance.
Molecular microbiology - 1 Jun 1991
Blázquez J, Davies J, Moreno F
Abstract excerpt
Aminoglycoside-phosphotransferases contain several conserved amino acid sequence motifs. Using hydroxylamine we have obtained five independent missense mutations within the aphA-2 gene of transposon Tn5. Four of the mutations dramatically reduced antibiotic resistance. Two were identical and incl...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Transposable Elements
- Escherichia coli
- Hydroxylamine
- Hydroxylamines
- Kanamycin
- Kanamycin Kinase
- Kanamycin Resistance
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Phosphorylation
- Phosphotransferases
- Plasmids
- Structure-Activity Relationship