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Article

Purification and characterization of aminoglycoside 3'-phosphotransferase type IIa and kinetic comparison with a new mutant enzyme.

Antimicrobial agents and chemotherapy - 1 Apr 1994

Siregar J J, Lerner S A, Mobashery S

Abstract excerpt

Aminoglycoside 3'-phosphotransferase [APH(3')s] provide an important means for high-level resistance to neomycin- and kanamycin-type aminoglycoside antibiotics. A four-step purification which affords milligram quantities of homogeneous APH(3') type IIa [APH(3')-IIa] is described. The kinetic parameters for the turnover of five substrates by the enzyme were determined, and the pH dependence and metal activation...

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Source

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Identifiers

PMID: 8031025
DOI: 10.1128/AAC.38.4.641
PMCID: PMC284518

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Source receipt: pubmed, retrieved 7 Sept 2026.

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