Article
Purification and characterization of aminoglycoside 3'-phosphotransferase type IIa and kinetic comparison with a new mutant enzyme.
Antimicrobial agents and chemotherapy - 1 Apr 1994
Siregar J J, Lerner S A, Mobashery S
Abstract excerpt
Aminoglycoside 3'-phosphotransferase [APH(3')s] provide an important means for high-level resistance to neomycin- and kanamycin-type aminoglycoside antibiotics. A four-step purification which affords milligram quantities of homogeneous APH(3') type IIa [APH(3')-IIa] is described. The kinetic para...
Topics
- Amino Acids
- Chromatography, Ion Exchange
- Cysteine
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Hydrogen-Ion Concentration
- Kanamycin Kinase
- Kinetics
- Metals
- Molecular Weight
- Mutation
- Phosphotransferases (Alcohol Group Acceptor)
- Spectrometry, Fluorescence