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Article

Mutant APH(2'')-IIa enzymes with increased activity against amikacin and isepamicin.

Antimicrobial agents and chemotherapy - 1 Apr 2010

Toth Marta, Frase Hilary, Chow Joseph W, Smith Clyde, Vakulenko Sergei B

Abstract excerpt

Directed evolution by random PCR mutagenesis of the gene for the aminoglycoside 2''-IIa phosphotransferase generated R92H/D268N and N196D/D268N mutant enzymes, resulting in elevated levels of resistance to amikacin and isepamicin but not to other aminoglycoside antibiotics. Increases in the activities of the mutant phosphotransferases for isepamicin are the result of decreases in K(m) values, while improved...

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Identifiers

PMID: 20145089
DOI: 10.1128/AAC.01444-09
PMCID: PMC2849353

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Source receipt: pubmed, retrieved 7 Sept 2026.

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