Article
A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.
Proceedings of the National Academy of Sciences of the United States of America - 1 May 1990
Yenofsky R L, Fine M, Pellow J W
Abstract excerpt
The neo (neomycin-resistance) gene of transposon Tn5 encodes the enzyme neomycin phosphotransferase II (EC 2.7.1.95), which confers resistance to various aminoglycoside antibiotics, including kanamycin and G418. The gene is widely used as a selectable marker in the transformation of organisms as diverse as bacteria, yeast, plants, and animals. We found a mutation that involves a glutamic to aspartic acid...
Topics
- Anti-Bacterial Agents
- Cells, Cultured
- Chimera
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Genetic Vectors
- Kanamycin Kinase
- Mutation
- Phosphotransferases
- Plants, Toxic