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Article

A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.

Proceedings of the National Academy of Sciences of the United States of America - 1 May 1990

Yenofsky R L, Fine M, Pellow J W

Abstract excerpt

The neo (neomycin-resistance) gene of transposon Tn5 encodes the enzyme neomycin phosphotransferase II (EC 2.7.1.95), which confers resistance to various aminoglycoside antibiotics, including kanamycin and G418. The gene is widely used as a selectable marker in the transformation of organisms as diverse as bacteria, yeast, plants, and animals. We found a mutation that involves a glutamic to aspartic acid...

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Identifiers

PMID: 2159150
DOI: 10.1073/pnas.87.9.3435
PMCID: PMC53915

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