Article
Amino acid substitutions within the analogous nucleotide binding loop (P-loop) of aminoglycoside 3'-phosphotransferase-II.
The International journal of biochemistry - 1 Jan 1994
Kocabivik S, Perlin M H
Abstract excerpt
1. Oligonucleotide-directed mutagenesis of APH(3')-II was used to investigate the functions of key amino acids in the P-loop analogous motif of the enzyme. 2. The mutations of Gly205-->Glu, Gly210-->Ala and Arg211-->Pro considerably reduced the resistance of the resulting strains to KM and to rel...
Topics
- Amino Acid Sequence
- Amino Acids
- Base Sequence
- Blotting, Western
- Drug Resistance, Microbial
- Kanamycin Kinase
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Nucleotides
- Phenotype
- Phosphotransferases (Alcohol Group Acceptor)
- Protein Conformation