Article
A new type of insertion mutation in monkey cells: insertion accompanied by long target site duplication.
Molecular & general genetics : MGG - 1 Oct 1991
Ohira M, Bae Y S, Ikeda H
Abstract excerpt
We have developed a system for the detection of a new type of insertion mutation in mammalian cells. We have used a shuttle vector, plasmid pNK1, which contains the SV40 and pBR322 replication origins, and ApR, galK, and neoR genes. This plasmid was introduced into monkey COS1 cells, allowed to r...
Topics
- Animals
- Base Sequence
- Cell Line
- DNA
- DNA Transposable Elements
- DNA, Satellite
- Haplorhini
- Molecular Sequence Data
- Mutation
- Plasmids
- Recombination, Genetic
- Repetitive Sequences, Nucleic Acid
- Restriction Mapping
- Sequence Homology, Nucleic Acid
- Transfection