Article
Design of a selectable reporter for the detection of mutations in mammalian simple repeat sequences.
Carcinogenesis - 1 May 1995
Kahn S M, Klein M G, Jiang W, Xing W Q, Xu D B, Perucho M, Weinstein I B
Abstract excerpt
To study the mutator phenotype characteristic of tumors showing widespread replication errors at simple DNA repeat sequences (RER+), we designed a selectable reporter system for the detection of such mutations in mammalian cells. A hygromycin B phosphotransferase gene was rendered out-of-frame by the insertion of a (CA)13 dinucleotide repeat tract immediately following the ATG start codon, and subcloned into a...
Topics
- 3T3 Cells
- Animals
- Base Sequence
- Cell Line
- Cloning, Molecular
- Codon
- Colonic Neoplasms
- DNA Primers
- DNA Replication
- Frameshift Mutation
- Humans
- Mammals