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Article

A shuttle vector for analysis of illegitimate recombination in mammalian cells: effects of DNA topoisomerase inhibitors on deletion frequency.

Gene - 30 May 1991

Bae Y S, Chiba M, Ohira M, Ikeda H

Abstract excerpt

To study the mechanism of illegitimate recombination in mammalian cells, we have developed a shuttle vector, pNK1, that contains three bacterial markers, amp (ApR), galK, and neo (KmR). The frequency of deletions occurring in autonomously replicating pNK1 DNA during the growth of monkey COS1 cells was measured by transfecting the plasmid into Escherichia coli cells and counting the number of galK- ApS double...

Topics

Amsacrine
Animals
Camptothecin
Cell Line
Chromosome Deletion
DNA Topoisomerases, Type II
Genetic Vectors
Mutation
Plasmids
Recombination, Genetic
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Teniposide

Topoisomerase II Inhibitors

Source

Identifiers

PMID: 1647363
DOI: 10.1016/0378-1119(91)90425-b

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1647363-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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