Article
Analysis of sequence specificity of 5-bromodeoxyuridine-induced reversion in cells containing multiple copies of a mutant gpt gene.
Somatic cell and molecular genetics - 1 Mar 1992
Rotstein J B, Kresnak M T, Samadashwily G M, Davidson R L
Abstract excerpt
For studies on molecular mechanisms of mutagenesis, it would be advantageous to transfer mutant genes with specific alterations into mammalian cells and use the transformed cells in reversion analyses. In the present paper, we describe an efficient method for analyzing reversion events occurring in cells that possess multiple copies of a mutational target gene. This method involves amplification of the...
Topics
- Animals
- Base Sequence
- Bromodeoxyuridine
- CHO Cells
- Cricetinae
- DNA
- DNA Restriction Enzymes
- Molecular Sequence Data
- Multigene Family
- Mutation
- Pentosyltransferases