Article
Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT-) using an Epstein-Barr virus-derived cDNA expression vector.
Nucleic acids research - 25 Sept 1991
Belt P B, Jongmans W, de Wit J, Hoeijmakers J H, van de Putte P, Backendorf C
Abstract excerpt
Human cells are, in general, poor recipients of foreign DNA, which has severely hampered the cloning of genes by direct phenotypic correction of deficient human cell lines after DNA mediated gene transfer. In this communication a methodology is presented which largely circumvents this problems. The method relies on the use of a recently developed episomal Epstein-Barr-virus-derived cDNA expression vector (Belt et...
Topics
- Base Sequence
- Cloning, Molecular
- DNA
- Drug Resistance
- Genetic Vectors
- Herpesvirus 4, Human
- Humans
- Hygromycin B
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data