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Article

Expression of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in E. coli.

Advances in experimental medicine and biology - 1 Jan 1991

Davidson B L, Roessler B J, Palella T D

Abstract excerpt

1. ECPR is a rapid and effective means for generating recombinant human HPRT. 2. The Bl21 (DE3) T7 polymerase/T7 promoter system provides high level expression of human HPRT constructs after induction of the T7 polymerase gene with IPTG. 3. Human HPRT constructs expressed in E. coli mimic the variant properties originally demonstrated in lymphoblast extracts from affected individuals. 4. Human HPRT expressed in...

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Identifiers

PMID: 1781351
DOI: 10.1007/978-1-4615-7703-4_23

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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