Article
Expression of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in E. coli.
Advances in experimental medicine and biology - 1 Jan 1991
Davidson B L, Roessler B J, Palella T D
Abstract excerpt
1. ECPR is a rapid and effective means for generating recombinant human HPRT. 2. The Bl21 (DE3) T7 polymerase/T7 promoter system provides high level expression of human HPRT constructs after induction of the T7 polymerase gene with IPTG. 3. Human HPRT constructs expressed in E. coli mimic the var...
Topics
- Base Sequence
- Cloning, Molecular
- DNA
- Escherichia coli
- Gene Expression
- Genetic Variation
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction