Article
Short DNA fragments induce site specific recombination in mammalian cells.
Molecular and cellular biochemistry - 9 Feb 1990
Hunger-Bertling K, Harrer P, Bertling W
Abstract excerpt
A defective hprt gene was corrected by homologous recombination in a lymphocyte cell line deficient in Hypoxanthine-phosphoribosyl-transferase activity (hprt). In a novel approach, only a fragment of a cDNA clone of the functional hprt gene was used to induce homologous recombination. The mutation that was corrected corresponds to a single base change in exon III of the hprt gene. Two transfection methods,...
Topics
- Amino Acid Sequence
- Base Sequence
- Capsid
- Cell Line
- DNA
- Exons
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Lymphocytes
- Molecular Sequence Data
- Mutation
- Plasmids