Article
Analysis of a temperature-sensitive vaccinia virus mutant in the viral mRNA capping enzyme isolated by clustered charge-to-alanine mutagenesis and transient dominant selection.
Virology - 24 Nov 1997
Hassett D E, Lewis J I, Xing X, DeLange L, Condit R C
Abstract excerpt
We have previously reported the successful development of a targeted genetic method for the creation of temperature-sensitive vaccinia virus mutants [D. E. Hassett and R. C. Condit (1994) Proc. Natl. Acad. Sci. USA 91, 4554-4558]. This method has now been applied to the large subunit of the multi...
Topics
- Alanine
- Amino Acid Sequence
- Cell Line
- Methyltransferases
- Molecular Sequence Data
- Morphogenesis
- Multienzyme Complexes
- Mutagenesis, Site-Directed
- Nucleotidyltransferases
- Phenotype
- Phosphoric Monoester Hydrolases
- Protein Processing, Post-Translational
- RNA, Messenger
- RNA, Viral