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Article

Directed evolution and substrate specificity profile of homing endonuclease I-SceI.

Journal of the American Chemical Society - 22 Feb 2006

Doyon Jeffrey B, Pattanayak Vikram, Meyer Carissa B, Liu David R

Abstract excerpt

The laboratory evolution of enzymes with tailor-made DNA cleavage specificities would represent new tools for manipulating genomes and may enhance our understanding of sequence-specific DNA recognition by nucleases. Below we describe the development and successful application of an efficient in vivo positive and negative selection system that applies evolutionary pressure either to favor the cleavage of a desired...

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Identifiers

PMID: 16478204
DOI: 10.1021/ja057519l

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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