Article
Loss of pseudorabies virus thymidine kinase activity due to a single base mutation and amino acid substitution.
The Journal of general virology - 1 Jun 1991
Prieto J, Martín Hernández A M, Tabarés E
Abstract excerpt
The nucleotide sequences of the coding region of the thymidine kinase (TK) gene of pseudorabies virus strain NIA3 and a TK- mutant (ATK5) were determined. The coding region of the TK gene consists of 320 codons capable of producing a polypeptide with an Mr of 34979. The mutant expressed an inacti...
Topics
- Amino Acid Sequence
- Animals
- Aspartic Acid
- Base Sequence
- Cell Line
- Cloning, Molecular
- Codon
- Genes, Viral
- Glycine
- Herpesvirus 1, Suid
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Protein Biosynthesis
- Restriction Mapping
- Sequence Homology, Nucleic Acid
- Thymidine Kinase
- Viral Structural Proteins