Article
Escherichia coli thymidylate synthase: amino acid substitutions by suppression of amber nonsense mutations.
Proceedings of the National Academy of Sciences of the United States of America - 1 May 1990
Michaels M L, Kim C W, Matthews D A, Miller J H
Abstract excerpt
By using site-directed oligonucleotide mutagenesis, amber nonsense stop codons (5'-TAG-3') have been introduced at 20 sites in the Escherichia coli thymidylate synthase gene. By transforming the thyA mutant plasmids into 13 strains, each of which harbor different amber suppressor tRNAs, we were able to generate over 245 amino acid substitutions in E. coli thymidylate synthase (EC 2.1.1.45). Growth characteristics...
Topics
- Amino Acid Sequence
- Amino Acids
- Escherichia coli
- Genes, Bacterial
- Models, Molecular
- Mutation
- Plasmids
- Protein Conformation
- Restriction Mapping
- Suppression, Genetic
- Thymidylate Synthase