A glycoprotein gX-beta-galactosidase fusion gene as insertional marker for rapid identification of pseudorabies virus mutants.

doi:10.1016/0166-0934(90)90043-f

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Article

A glycoprotein gX-beta-galactosidase fusion gene as insertional marker for rapid identification of pseudorabies virus mutants.

Journal of virological methods - 1 Oct 1990

Mettenleiter T C, Rauh I

Abstract excerpt

We describe the isolation and characterization of infectious pseudorabies virus (PrV) mutants expressing functional beta-galactosidase. To obtain high level expression of the enzyme, sequences of the bacterial beta-galactosidase gene starting with codon 8 were inserted in frame behind the promote...

Topics

Animals
Cell Line
DNA, Viral
Genes, Viral
Genetic Markers
Herpesvirus 1, Suid
Mutagenesis, Insertional
Mutation
Recombinant Fusion Proteins
Restriction Mapping
Transfection
Viral Envelope Proteins
beta-Galactosidase

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