Article
Isolation of a viable herpesvirus (pseudorabies virus) mutant specifically lacking all four known nonessential glycoproteins.
Virology - 1 Nov 1990
Mettenleiter T C, Kern H, Rauh I
Abstract excerpt
Recently we described the isolation and characterization of a pseudorabies virus (PrV) mutant lacking the nonessential glycoproteins gI, gp63, and gIII. Using insertional mutagenesis with a functional gX-beta-galactosidase fusion gene we describe here the isolation of a PrV mutant specifically lacking all four known nonessential glycoproteins, gI, gp63, gIII, and gX. The quadruple mutant did not show any...
Topics
- Animals
- Blotting, Southern
- Cell Line
- Chromosome Deletion
- DNA, Viral
- Genes, Viral
- Glycoproteins
- Herpesvirus 1, Suid
- Molecular Weight
- Mutation
- Protein Biosynthesis
- RNA, Viral