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Article

Construction and transposon mutagenesis in Escherichia coli of a full-length infectious clone of pseudorabies virus, an alphaherpesvirus.

Journal of virology - 1 Aug 1999

Smith G A, Enquist L W

Abstract excerpt

A full-length clone of the 142-kb pseudorabies virus (PRV) genome was constructed as a stable F plasmid in Escherichia coli. The clone, pBecker1, was colinear with PRV-Becker genomic DNA, lacking detectable rearrangements, deletions, or inversions. The transfection of pBecker1 into susceptible eukaryotic cells resulted in productive viral infection. Virus isolated following transfection was indistinguishable from...

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Identifiers

PMID: 10400733
DOI: 10.1128/JVI.73.8.6405-6414.1999
PMCID: PMC112720

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Source receipt: pubmed, retrieved 7 Sept 2026.

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