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Article

[Construction and characterization of a pseudorabies virus TK-/gG- mutant].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Jul 2004

Xu Xiao-Juan, Xu Gao-Yuan, Chen Huan-Chun, Liu Zheng-Fei, He Qi-Gai

Abstract excerpt

To construct a TK-/gG- mutant of pseudorabies virus, the gG-detected transfer vector pUSKKBB and genomic DNA of pseudorabies virus TK-/gG-/LacZ+ were co-transfected into IBRS-2 cells. Transfection progeny were plated onto PK-15 cells and incubated for 2 days under methylcellulose. Then the overlay was removed and replaced by 1% low melting point agarose in DMEM supplemented with 150 microg/mL X-gal. After 2 days,...

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PMID: 15968983

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