Article
Identification of important residues within the putative nucleoside binding site of HSV-1 thymidine kinase by random sequence selection: analysis of selected mutants in vitro.
Biochemistry - 2 Nov 1993
Black M E, Loeb L A
Abstract excerpt
Random sequence mutagenesis in conjunction with genetic complementation was used to map the function of amino acid residues within the putative nucleoside binding site of the herpes simplex virus type 1 (HSV-1) thymidine kinase (TK). Six codons of the putative nucleoside binding site of the HSV-1...
Topics
- Animals
- Base Sequence
- Binding Sites
- Consensus Sequence
- Enzyme Stability
- Herpesvirus 1, Human
- Molecular Sequence Data
- Mutation
- Nucleosides
- Rabbits
- Recombinant Proteins
- Substrate Specificity
- Thymidine Kinase