Article
Amino acid substitution in the lactose carrier protein with the use of amber suppressors.
Journal of bacteriology - 1 Aug 1992
Huang A M, Lee J I, King S C, Wilson T H
Abstract excerpt
Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid substitutions in the regions of the putative loops (between transmembrane alpha helices) resulted...
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