Amino acid substitution in the lactose carrier protein with the use of amber suppressors.

doi:10.1128/jb.174.16.5436-5441.1992

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Article

Amino acid substitution in the lactose carrier protein with the use of amber suppressors.

Journal of bacteriology - 1 Aug 1992

Huang A M, Lee J I, King S C, Wilson T H

Abstract excerpt

Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Mo...

Topics

Amino Acid Sequence
Amino Acids
Base Sequence
Cloning, Molecular
Escherichia coli
Escherichia coli Proteins
Immunohistochemistry
Lactose
Melibiose
Membrane Transport Proteins
Methylgalactosides
Molecular Sequence Data
Monosaccharide Transport Proteins
Mutation
Suppression, Genetic
Symporters
Thiogalactosides

Source

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Identifiers

PMID: 1644770
DOI: 10.1128/jb.174.16.5436-5441.1992
PMCID: PMC206383

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1644770-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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