Article
A simple screen for permissive sites in proteins: analysis of Escherichia coli lac permease.
Journal of molecular biology - 28 Mar 1997
Manoil C, Bailey J
Abstract excerpt
Proteins can be remarkably tolerant of major mutational changes. Sites that accomodate large insertions without loss of function ("permissive" sites) appear generally to correspond to surface regions at which the added sequences do not disrupt overall folding. The identification of such sites can...
Topics
- Amino Acid Sequence
- Base Sequence
- Biological Transport
- Blotting, Western
- Cloning, Molecular
- DNA Methylation
- DNA Transposable Elements
- DNA, Recombinant
- Escherichia coli
- Escherichia coli Proteins
- Genes, Reporter
- Lactose
- Membrane Proteins
- Membrane Transport Proteins
- Molecular Sequence Data
- Monosaccharide Transport Proteins
- Mutagenesis, Insertional
- Mutagenesis, Site-Directed