Characterization and sequencing of an uncoupled lactose carrier mutant of Escherichia coli.

doi:10.1006/bbrc.1994.1444

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Article

Characterization and sequencing of an uncoupled lactose carrier mutant of Escherichia coli.

Biochemical and biophysical research communications - 15 Apr 1994

Matos M E, Wilson T H

Abstract excerpt

A lactose carrier mutant of Escherichia coli (ML308-22) showed a severe defect in thiomethylgalactoside accumulation but a faster than normal entry of o-nitrophenyl-galactoside. Sequencing of the mutant lacY gene revealed a point mutation resulting in the substitution of glycine-159 by a cysteine residue. The mutant showed an increased sensitivity to sulfhydryl reagents, a property that is consistent with the...

Topics

Amino Acid Sequence
Base Sequence
Biological Transport
DNA Primers
Escherichia coli
Escherichia coli Proteins
Genes, Bacterial
Kinetics
Lactose
Membrane Transport Proteins
Methylgalactosides

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Models, Structural

Molecular Sequence Data

Monosaccharide Transport Proteins

Mutation

Polymerase Chain Reaction

Protein Structure, Secondary

Symporters

Source

Open in PubMed

Identifiers

PMID: 8166695
DOI: 10.1006/bbrc.1994.1444

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8166695-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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