Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Characterization and sequencing of an uncoupled lactose carrier mutant of Escherichia coli.

Biochemical and biophysical research communications - 15 Apr 1994

Matos M E, Wilson T H

Abstract excerpt

A lactose carrier mutant of Escherichia coli (ML308-22) showed a severe defect in thiomethylgalactoside accumulation but a faster than normal entry of o-nitrophenyl-galactoside. Sequencing of the mutant lacY gene revealed a point mutation resulting in the substitution of glycine-159 by a cysteine residue. The mutant showed an increased sensitivity to sulfhydryl reagents, a property that is consistent with the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Base Sequence
Share to DNA Primers

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Escherichia coli

Share to Genes, Bacterial

Share to Molecular Sequence Data

Share to Mutation

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 8166695
DOI: 10.1006/bbrc.1994.1444

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Characterization and sequencing of an uncoupled lactose carrier mutant of Escherichia coli.PMID 8166695Read ↗

Identification of valine 177 as a mutation altering specificity for transport of sugars by the Escherichia coli lactose carrier. Enhanced specificity for sucrose and maltose.Semantic proximity Read ↗

Molecular biology of the lactose carrier of Escherichia coli.Semantic proximity Read ↗

Towards an understanding of the structural basis of 'forbidden' transport pathways in the Escherichia coli lactose carrier: mutations probing the energy barriers to uncoupled transport.Semantic proximity Read ↗

Lactose transport mutants of Escherichia coli resistant to inhibition by the phosphotransferase system.Semantic proximity Read ↗

An analysis of suppressor mutations suggests that the two halves of the lactose permease function in a symmetrical manner.Semantic proximity Read ↗

Suppressor analysis of mutations in the loop 2-3 motif of lactose permease: evidence that glycine-64 is an important residue for conformational changes.Semantic proximity Read ↗

A single nucleotide polymorphism in ycdC alters tRNA synthetase expression and results in hypersecretion in Escherichia coli.Semantic proximity Read ↗

Regulation of the glyoxylate bypass operon: cloning and characterization of iclR.Semantic proximity Read ↗

Characterization of Escherichia coli lactose carrier mutants that transport protons without a cosubstrate. Probes for the energy barrier to uncoupled transport.Semantic proximity Read ↗

Cysteine-scanning mutagenesis of helix II and flanking hydrophilic domains in the lactose permease of Escherichia coli.Semantic proximity Read ↗

An Escherichia coli mutant conditionally altered in respiratory chain components.Semantic proximity Read ↗

Evidence for an intermediate conformational state of LacY.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.