Article
Use of amber suppressors to investigate the thermostability of Bacillus licheniformis alpha-amylase. Amino acid replacements at 6 histidine residues reveal a critical position at His-133.
The Journal of biological chemistry - 15 Sept 1990
Declerck N, Joyet P, Gaillardin C, Masson J M
Abstract excerpt
A set of 12 Escherichia coli suppressor tRNAs, inserting different amino acids in response to an amber codon, has been used to create rapidly numerous protein variants of a thermostable amylase; by site-directed mutagenesis, amber mutations were first introduced into Bacillus licheniformis alpha-...
Topics
- Amino Acid Sequence
- Bacillus
- Cloning, Molecular
- Enzyme Stability
- Genes, Bacterial
- Histidine
- Molecular Sequence Data
- Mutation
- Plasmids
- Sequence Homology, Nucleic Acid
- Suppression, Genetic
- Thermodynamics
- alpha-Amylases