Article
Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence.
Journal of molecular biology - 29 Jul 1994
Markiewicz P, Kleina L G, Cruz C, Ehret S, Miller J H
Abstract excerpt
Amber mutations have been constructed at 328 positions, corresponding to residues 2 to 329 in the E. coli lac repressor protein. Synthetic and naturally occurring nonsense suppressors have been used to insert, in series, 12-13 amino acids at positions specified by an amber (UAG) codon in the lacI...
Topics
- Alanine
- Amino Acid Sequence
- Amino Acids
- Bacterial Proteins
- Enzyme Induction
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Lac Repressors
- Molecular Sequence Data
- Mutation
- Phenotype
- Protein Structure, Secondary
- Repressor Proteins