Article
The melibiose carrier of Escherichia coli: cysteine substitutions for individual residues in helix XI.
The Journal of membrane biology - 15 Mar 2000
Ding P Z, Wilson T H
Abstract excerpt
The melibiose carrier from Escherichia coli is a sugar-cation cotransport system. Previously evidence was obtained that this integral membrane protein consists of 12 transmembrane helices. Starting with the cysteine-less melibiose carrier, cysteine has been substituted individually for amino acids 374-396, which includes all of the residues in the proposed helix XI. The carriers with cysteine substitutions were...
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