Article
Detection of mononucleotide repeat sequence alterations in a large background of normal DNA for screening high-frequency microsatellite instability cancers.
Clinical cancer research : an official journal of the American Association for Cancer Research - 15 Jan 2006
Sun Xiyuan, Liu Yiding, Lutterbaugh Jim, Chen Wei-Dong, Markowitz Sanford D, Guo Baochuan
Abstract excerpt
PURPOSE: Mutations in mononucleotide repeat sequence (MRS) are good indicators of high-frequency microsatellite instability (MSI-H) cancers, but it has been a challenge to detect such mutations in a large background of wild-type DNA; as in this setting, PCR errors often generate false positive mutant alleles. In this study, we developed a general strategy, referred to as probe clamping primer extension-PCR...
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