Article

Microsatellite Instability Detection in Clinical Cancer Samples: A Multiplex qPCR Approach without Matching Normal Samples

2023-11-08

Abstract excerpt

<h4>Background</h4> Microsatellite instability (MSI) indicates DNA mismatch repair deficiency in cancers like colorectal cancer. The current gold standard technique, PCR/capillary electrophoresis (CE), requires matching normal samples and specialized instrumentation. We developed VarTrace, a rapid and low-cost quantitative PCR (qPCR) assay, to evaluate MSI using solely the tumor sample DNA, obviating the requireme...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d64c9684-a340-5c2f-9aed-494066c94554
DOI: 10.1101/2023.11.07.23298217

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Microsatellite Instability Detection in Clinical Cancer Samples: A Multiplex qPCR Approach without Matching Normal SamplesDOI 10.1101/2023.11.07.23298217Read

Improved NGS-based detection of microsatellite instability using tumor-only dataSemantic proximity Read

Detection of Microsatellite Instability: State of the Art and Future Applications in Circulating Tumour DNA (ctDNA)Semantic proximity Read

Evaluation of 3 molecular-based assays for microsatellite instability detection in formalin-fixed tissues of patients with endometrial and colorectal cancersSemantic proximity Read

Sensitive detection of microsatellite instability in tissues and liquid biopsies: Recent developments and updatesSemantic proximity Read

Accurate Microsatellite Instability Classification Using Ultima Genomics Platform Across Multiple Tumor TypesSemantic proximity Read

Reference-free microsatellite instability detection from tumor sequencing using intrasample variability modelingSemantic proximity Read

Analytical Validation and Clinical Utilization of the Oncomine Comprehensive Assay Plus Panel for Comprehensive Genomic Profiling in Solid TumorsSemantic proximity Read

Real-World Evaluation of Microsatellite Instability Detection via Targeted NGS Panels in Routine Molecular DiagnosticsSemantic proximity Read

Accurate Pan-Cancer Molecular Diagnosis of Microsatellite Instability by Single-Molecule Molecular Inversion Probe Capture and High-Throughput SequencingSemantic proximity Read

MSIanalyzer: Targeted Nanopore Sequencing Enables Single Nucleotide Resolution Analysis of Microsatellite Instability Diversity.Semantic proximity Read

MSIsensor: microsatellite instability detection using paired tumor-normal sequence dataSemantic proximity Read

Epidemiology of Microsatellite Instability High (MSI-H) and Deficient Mismatch Repair (dMMR) in Solid Tumors: A Structured Literature ReviewSemantic proximity Read